Micro-behavior of pilots is one of the most remarkable aspects in flight safety research domain. The study of pilot’s micro-behavior and its function are of great significance to enhance active safety warnings of flight and evaluation of flight cadets. Based on the cognitive process of pilots, this paper explores the meanings and contents of previous research on the pilot’s micro-behavior. The history and research status of pilot’s micro-behavior are briefly introduced from the perspective of their psychology, physiology and physics. The current reviews mainly include the pilot’s characteristic, multi-information fusion, integrated cognitive and humanization about controlling environment, etc. The several methods of these studies are discussed, and the mechanisms, experimental contents and applicable conditions of pilot’s physiological, psychological and physical characteristics are analyzed. Meanwhile, the advantages and shortcomings of the existing research results are pointed out and analyzed. Combined with flight simulation experiment, the internal mechanism of pilot is explained. Furthermore, with the latest research in the modern flight field, and also from the specialization of application, the diversification of methodologies and the depth of investigation are provided, as well as the development trend of pilot’s micro-behavior analysis in the future.